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1.What is the Fluorescein isothiocyanate ?

Dark yellow powder, soluble in water and decomposed in water. It is easily soluble in alkaline aqueous solution, and the strong green fluorescence disappears and precipitates. After adding alkali, it dissolves and shows fluorescence. Insoluble in acetone, ether and petroleum ether. Hygroscopic.

ChEBI: Fluorescein 5-isothiocyanate is the 5-isomer of fluorescein isothiocyanate. Acts as a fluorescent probe capable of being conjugated to tissue and proteins; used as a label in fluorescent antibody staining procedures as well as protein- and amino acid-binding techniques.

Fluorescein isothiocyanate (FITC) is an amine-reactive derivative of fluorescein dye that has wide-ranging applications as a label for antibodies and other probes, for use in fluorescence microscopy, flow cytometry and immunofluorescence-based assays such as Western blotting and ELISA. The isothiocyanate variety of fluorescein efficiently labels antibodies and other purified proteins at primary amines (lysine side chains)Excitation source: 488 nm spectral line, argon-ion laserExcitation wavelength: 494 nmEmission wavelength: 518 nmExtinction coefficient: > 70,000 M-1cm-1Solubility: Soluble in aqueous buffers at pH > 6Reactive groups: Isothiocyanate, reacts with primary amines at pH 7.0 to 9.0

2.What is the CAS number for Fluorescein isothiocyanate ?

The CAS number of Fluorescein isothiocyanate is 27072-45-3.

3.What are another words for Fluorescein isothiocyanate ?

Synonyms?for?Fluorescein isothiocyanate 27072-45-3:Fluorescein,isothiocyanato- (6CI,8CI); FITC; Fluorescein isothiocyanate

4.What is Fluorescein isothiocyanate (27072-45-3) used for?

For fluorescent labeling of proteins (e.g. immunofluorescence); H Protein labeling for gel chromatography.Label antibodies for use as immunofluorescent probes.Label oligonucleotides for hybridization probes.Detect proteins in gels and on Western blots.

InChI:InChI=1/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H

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